General Cell Based Assays
Epigentek Inc Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric) (48 Assays)
For measurement of total activity/inhibition of m6A methylases from nuclear extracts or purified enzymes
Eurofins Discoverx PathHunter CNR1 Bioassay Kit, 10-Plate. (93-0959Y2-00120)
The PathHunter CNR1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. CP 55,940) based activation of CNR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CNR1 and EA-tagged β-Arrestin-2. Activation of CNR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress TORC1 Nuclear Translocation Assay, 10pl Kit. (93-0833E3CP19L)
The PathHunter eXpress TORC1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the CRTC/CREB pathway. This kit assesses ligand (e.g. forskolin) based pathway activation via detection of resulting TORC1 (CRTC1) nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged TORC1 (CRTC1) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam L-Lactate Assay Kit (Colorimetric/Fluorometric)
L-Lactate Assay Kit (Colorimetric/Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress OPRD1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0400E2CP2L)
The PathHunter OPRD1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. DADLE) based activation of OPRD1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRD1 and EA-tagged β-Arrestin-2. Activation of OPRD1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL7R/CRLF2 Dimerization Assay, 10pl Kit. (93-1019E13CP25L)
The PathHunter IL7R/CRLF2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TSLP) induced dimerization of IL7R/CRLF2 receptors. This product uses EFC technology. The cell line included overexpresses IL7R target protein fused to PK alongside CRLF2 target protein fused to EA. Upon ligand-induced dimerization of IL7R/CRLF2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CCR6 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0069E2CP2M)
The cAMP Hunter CCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL20) based activation of CCR6 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CCR6, a naturally Gi/Go coupled GPCR. Upon activation of CCR6, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter LHCGR Bioassay Kit, 10-Plate. (95-0106Y2-00138)
The cAMP Hunter CHO-K1 LHCGR Bioassay Kit provides a robust, and highly sensitive functional, cell-based assay to measure drug potency and detect neutralizing antibodies.. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility and faster implementation from characterization to lot release. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter ProLabel Detection Kit, 10,000 dp (384-well). (93-0180L)
The PathHunter ProLabel Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter cells overexpressing ED (ProLabel/PK) but not containing any EA. This kit contains lysis buffer, exogenous EA, and EFC substrate to allow for detection of levels of ED in the assayed cells. This kit contains all components required to assay 25 plates (384-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0180l.
BioVendor, LLC QuickZyme Sensitive Tissue Collagen Assay Kit 96-Assays
QuickZyme Sensitive Tissue Collagen Assay Kit, 96-Assays. Measure hydroxyproline in small tissue samples with low collagen levels! The ONLY hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate, enabling measurement in small samples with low hydroxyproline levels, such as liver. No drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay. Dynamic range: 6-300 ug/ml. Sensitivity: 2.5 ug/ml. Sample vol. 50-500 ul. Species independent. Measures total hydroxyproline. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all collagen types present without discriminating between types of collagen, procollagen, mature collagen and collagen degradation products. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. Collagen hydrolysis tubes & protocol ARE included. Ease-of-use: Equivalent to ELISA.
Montana Molecular Borealis Arrestin GCGR Assay - 10mL
Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Glucagon receptor (GCGR) assay. Add the unmodified GCGR and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, GCGR BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). Borealis kits for several other receptors are also available, and larger-sized kits are available on request. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.
TRIBIOSCIENCE INC Mitochondrial Complex III Activity Colorimetric Assay 100 Tests
The activity of Complex III is directly measured through the increase of reduction of cytochrome c at absorbance of 550nm. This kit uses cytochrome c as substrate. The reduced cytochrome c is determined by recording the change in absorbance (A550). Applications Complex III Activity.
Bioassay Systems EnzyChrom™ Adipolysis Assay Kit. For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.
For quantitative assay of adipolysis and evaluation of drug effects on adipolysis. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.92 to 100 µg/mL (10 to 1000 µM) glycerol for colorimetric assays and 0.2 to 5 µg/mL for fluorimetric assays. Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates. Method: OD570nm, or FL530/585nm. Samples: Cell culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 200 tests. Detection limit: 0.92 µg/mL.
Epigentek Inc Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric) (96 assays)
The Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric) is a complete set of optimized buffers and reagents for measuring activity/inhibition of total cytosine oxygenase TET enzymes in nuclear extracts or purified TET isoforms (TETs 1-3) from a broad range of species such as mammals, plants, fungi, and bacteria, and in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues.